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Image Search Results
Journal: bioRxiv
Article Title: Memory B cells dominate the early antibody-secreting cell response to SARS-CoV-2 mRNA vaccination in naïve individuals independently of their antibody affinity
doi: 10.1101/2023.12.01.569639
Figure Lengend Snippet: a , Study design. I – first dose; II – second dose; pre-I and pre-II – the day before the first and the second vaccination; 1w, 2w, 3w – one, two and three weeks after vaccination. b , Frequency of S+ B cells in memory (CD19+CD27+) and naïve (CD19+CD27-) population over time. c , Frequency of ASCs over time. d , UMAP projection of peripheral blood B cells transcriptional profiles colored by population defined by transcriptome analysis. ASC – antibody-secreting cell; MBC – memory B cell. e , Expression profile of selected marker genes. f, IGHV + IGK/LV somatic hypermutation (SHM) counts across the cell populations. g , Class-switched antibody frequency. h , Clone size distribution. i-j , Frequency of CD71+ cells in S+ ( i ) and S-( j ) naïve (CD19+CD27-) and memory B cells (CD19+CD27+). k-l , Frequency of antibody secreting ( k ) and B cell ( l ) populations in S+ cells over time. Dots show average frequency across the volunteers and error bars show SEM. Dotted vertical lines indicate prime and boost.
Article Snippet: Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen, 1:200), CD8-APC-eFluor 780 (Invitrogen, 1:200), CD14-APC-eFluor 780 (Invitrogen, 1:200), CD16-APC-eFluor 780 (Invitrogen, 1:200), CD27-PE-Cy7 (BioLegend, 1:20), CD38-BV605 (BD Biosciences, 1:20),
Techniques: Expressing, Marker